Technologies

Customized Plant Bioink Composition & 3D Bioprinted scaffolds for biomedical applications


Abstract/Technology Overview

Our Technology

Proprietary triple technology of plant bioactive extraction, plant bioink composite and 3D bioprinting methodology, enables 3D bioprinting of high resolution, multi-layered bioscaffold with larger surface area, smaller and controllable pore sizes (400-100 micron or smaller)  and greater porosity (>90%). Bioscaffolds printed have demonstrated enhanced cell attachment and proliferation, tunable biodegradation profile that can be monitored in vivo and non-invasively in real time. Bioscaffold is also capable of encapsulating bioactives to be released in a controlled manner as the bioscaffold biodegrades - ideal for therapeutic applications such as wound dressing.

 

3D Bioprinting Market Challenges

  • Limited supply of bioink that is printable and has high cytoaffinity.
  • Collagen, a popular source of natural bioink polymer, is expensive and poses risk of immunogenicity and disease transmission from animals.
  • Different biomedical applications need bioscaffold with different properties but marketed bioscaffold has standard properties which cannot be used in many biomedical applications

Our Solution

  • Extractions of plant protein for formulation into plant bioink composite offer an economical source of natural protein bioink composite.
  • Our plant bioink composite formulation is economical with printing properties that can be customized for different bioprinters.  
  • Our plant bioink can also be molecularly tagged to encapsulate therapeutics.
  • Our 3D bioprinted bioscaffold has enhanced bioaffinity, enhanced biodegradation which can be monitored in-vivo in real time. Properties of our 3D bioprinted bioscaffold can be tuned in accordance to specifications for different biomedical applications and tissue types.

 


Technology Features, Specifications and Advantages

3D bioprinted bioscaffolds with the described proprietary plant derived bioink and 3D printing methodology, have the following advantages:

  1. Customizable
  2.  Bioink can be customized to different 3D bioprinters and to different biomedical applications
  3.  Bioscaffold design and diversity of structural fabrication can be customized in accordance to required specifications for different biomedical applications and tissue types
  4. Tunable biodegradation profile
  5. Cytocompatible
  6. Batch to batch reproducibility
  7. Enhanced cell affinity and proliferation
  8. Real time, non-invasive monitoring of biodegradation profile in-vivo
  9. Controlled release of bioactives encapsulated in the plant bioink
  10. Plant bioink is more economical and has lower risk profile than collagen, and other animal proteins.

Jing, L., Wang, X., Liu, H., Lu, Y., Bian, J., Sun, J., & Huang, D. (2018). Zein Increases the Cytoaffinity and Biodegradability of Scaffolds 3DPrinted with Zein and Poly(ε-caprolactone) Composite Ink. Applied Materials & Interfaces.


Potential Applications

1.  For 3D Bioprinting Companies:   

  • Customized development of plant based bioink composite for use in different bio-printers and for different biomedical applications

2.  Customized 3D bioprinting of bioscaffolds in accordance to required specifications for:

  • Therapeutic applications e.g. wound dressing with controlled-release of medicine embedded in the scaffold.
  • Tissue engineering applications
  • Regenerative medicine applications
  • 3D Cell cultures for drug discovery and development
  • Tumour tissue model for cancer research

 


Customer Benefit

  • Companies and researchers who need bioscaffolds for 3D cell cultures, tumour models, regenerative medicine and tissue engineering development can have bioscaffolds customized and 3D bioprinted to the specifications needed without having to fork out large upfront capital investments to purchase different 3D bioprinters and time required to learn how to operate and experiment with the different means of printing bioscaffold of different specifications. The bioscaffold can be developed with customized bioink composite and 3D bioprinted in accordance to the design, tunable cell structures that will enhance performance of the customers’ products, as defined by the customers.
  • The greater printability and cell supportive function of the plant based bioink composite enables development of tissue cultures without the need for cells to be embedded into bioink and undergo stress of printing thereby minimizing cell damage/death.
  • Plant base bioink is more cost effective than current bioink that contains animal derived ink materials.
  • Batch by batch reproducibility of bioink and bioscaffold, unlike animal derived bioink
  • Able to encapsulate bioactives and non-toxicity.

In summary, the greatest benefit to customers is ability to have customized bioscaffold without the significant cost and risk of customization.

 

OVERVIEW
Technology Owner

Fiona Tan

Company

Kosmode Health Singapore

Technology Category
  • Diagnostics
  • Pharmaceuticals & Therapeutics
  • Biotech Research Reagents & Tools
  • Additive Manufacturing
  • Bio Materials
  • Composites (Polymer-Matrix)
Technology Status
  • Available for Licensing
Technology Readiness Level
  • TRL 9
Keywords

3D Bioprinting, Bioink, Biomaterial, Plant-derived protein, Bioscaffold, 3D cell culture, Biomedical